مقایسه تاثیرمواد پیوندی Totudentو Bio-oss بر روی فعالیت میتوکندری وآلکالین فسفاتازی سلول‌های شبه استئوبلاست saos-2

Abstract Background: Several reports on clinical utility of various Bone Substitute Materials, (BSM) for grafting have shown successful results. The most important factor in successful grafting of BSM is the physiological and histological behavior of this material which can be evaluated by observing, rate of survival and proliferation of osteogenic cells. This research was conducted to examine the effect of Totudent and Bio-oss on the morphology, proliferation and rate of survival of Saos-2 osteoblastoid cells in vitro. Methods: This experimental study was done on two types of materials used as bone grafts viz. Totudent and Bio-oss. Twelve samples from each of these 2 materials grafted on Saos-2 osteoblastoid cells and 4 negative control samples were studied. On day 15, the rate of cell survival was determined by the MTT test and on day 20, Alkaline phosphatase activity of these cells in the culture medium were evaluated by the Alkaline phosphatase kit. Results: The rate of cell survival in the Bio-oss and Tutodent group was significantly diminished in comparison with the control group. This rate was significantly higher in the cells grafted with Tutodent as compared to Bio-oss alkaline phosphatase activity in Totudent group was higher than the other 2 groups. Conclusion: Tutodent bone graft showed better result in the morphologic variations of Saos-2 cells in the culture medium as compared to Bio-oss, and has more appropriate biologic characteristics. Keywords: Bone replacement material, Cell line Saos-2, Cell viability, Alkaline phosphatase.